Prothrombin Activation by Prothrombinase in a Tubular Flow Reactor
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چکیده
منابع مشابه
Activation of Human Prothrombin by Human Prothrombinase
The kinetics of the activation of human prothrombin catalyzed by human prothrombinase was studied using the fluorescent a-thrombin inhibitor dansylarginineN-(3-ethyl-1,5-pentanediyl)amide(DAPA). Prothrombinase proteolytically activates prothrombin to athrombin by cleavages at Arg273-Thr274 (bond A) and Arg322-Ile323 (bond B). The differential fluorescence properties of DAPA complexed with the...
متن کاملFate of membrane-bound reactants and products during the activation of human prothrombin by prothrombinase.
Membrane binding by prothrombin, mediated by its N-terminal fragment 1 (F1) domain, plays an essential role in its proteolytic activation by prothrombinase. Thrombin is produced in two cleavage reactions. One at Arg(320) yields the proteinase meizothrombin that retains membrane binding properties. The second, at Arg(271), yields thrombin and severs covalent linkage with the N-terminal fragment ...
متن کاملSpecific contribution of different phospholipid surfaces to the activation of prothrombin by the fully assembled prothrombinase.
This paper addresses, in thermodynamic and kinetic terms, the reasons for the acidic lipid specificity of the human prothrombinase complex. We obtained, from the measured lipid titrations of the initial rates of prothrombin activation, the empirical binding constants for prothrombinase assembly on different membranes. These favored assembly on phosphatidylserine (PS)- as opposed to phosphatidyl...
متن کاملAn inhibitory monoclonal antibody to factor X that blocks prothrombin activation but not prothrombinase enzyme assembly.
A monoclonal antibody (designated alpha BFX-2b) prepared against bovine factor X inhibited factor X activity in human, bovine, porcine, rabbit, and canine plasma. In assays using purified prothrombinase components, factor Xa, factor Va, phospholipid vesicles, and calcium ion with the fluorescent active site thrombin inhibitor dansylarginyl-N-(3-ethyl-1,5-pentanediyl)amide, the antibody inhibite...
متن کاملThe activation of prothrombin by the prothrombinase complex. The contribution of the substrate-membrane interaction to catalysis.
The conversion of prothrombin to thrombin requires the cleavage of two peptide bonds and is catalyzed by the prothrombinase complex composed of factors Xa and Va assembled on a membrane surface. Presteady-state kinetic studies of the effects of membranes on the proteolytic reaction were undertaken using model membranes composed of phosphatidylcholine and phosphatidylserine (PCPS). The concentra...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1995
ISSN: 0021-9258
DOI: 10.1074/jbc.270.3.1029